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KMID : 0359319730130010047
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Korean Journal of Veterinary Research
1973 Volume.13 No. 1 p.47 ~ p.52
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Studies on the Constituents of Bordetella bronchiseptica
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Abstract
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An attempt was carried out to purify the cell components of Bordetella bronchiseptica by the mild procedures. From the supernatant of sonic treated colls, the K-agglutinogen, hemagglutinogen and skin necrotizing factor were separated by a successive use of DEAE-cellulose column chromatography and ammonium sulfate precipitation.
The specific activity of purified K-agglutinogen was demonstrated by a gel-diffusion test and was also found to be free from other biologically active substances of B. bronchiseptica: namely hemagglutinin, skin-necrotizing factor and O-antigen.
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